Artha Wacana Christian University

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Exploration study of the pigment types of stone crab (Grapsus albolineatus) as pharmaceutical and cosmetics Ingridients

        Darus S.J. Paransa; dkk ...;Alfread Kase; Anggrein,2019
Darus Saadah J. Paransa1*, Desy M.H. Mantiri1, James J.H. Paulus1, Cysca Lumenta1, Medy ompie1, Suria Darwisito1, Carolus Paruntu1, Alfread Kase2 and Anggreini D.N. Rupidara2 1Fisheries and Marine Science Study Program of Sam Ratulangi University, Manado, North Sulawesi, Indonesia 2Fisheries and Marine Science Study Program of Artha Christian University, Kupang, East Nusa Tenggara, Indonesia (Received 14 March, 2019; Accepted 27 May, 2019) ABSTRACT Grapsus albolineatus is a type of crustacean on the rocky coast of tropical regions like Indonesia. Some of the distribution areas of this crab species are the rocky coastline of, Tanawangko and Manggatasik Village of, Manado City, North Sulawesi. Grapsus albolineatus is known to have a high content of carotenoid pigment. Carotenoid pigments are useful for human needs, such as immune substances, and various industrial raw materials such as pharmaceutical, cosmetic and foodstuff industries. In this study, identification of the pigment type was performed on the carapace, gonad, blood, epidermal layer, and hepatopancreas using Thin Layer Chromatography method. The identification of pigments on the carapace was using Column Chromatography and High-Performance Liquid Chromatography (HPLC) methods. The results showed that Grapsus albolineatus was identified at the D molting stage. In the Thin Layer Chromatography analysis, there were found some pigment types* namely -Carotene, Echinenone, Canthaxanthin, Cryptoxanthin, Adonirubin, Astaxanthin and Astacen. Meanwhile, the separation by Column Chromatography method showed the results of pigments such as -Carotene, Canthaxanthin, Adonirubin, and Fucoxanthin. The concentrations of pigments contained in the carapace, epidermal layer, hepatopancreas, gonad and blood were respectively 6.88 mg/g, 210.47 mg/g, 454.0 mg/g, 433.33 mg/g, and 153 mg/g. The pigment content of each organ of G. albolineatus crabs was 6.95 mg (the carapace), 7.26 mg (epidermal layer), 7.07 mg (hepatopancreas), 34.94 mg (gonad), and 5.86 mg (hemocyanin). Key words : Pigment, Carotenoid, Crustacea, TLC, CC

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2019 EEC-1.Member -Exploration Study of the pigment types.pdf

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