SCIENTIFIC REPOSITORY

Artha Wacana Christian University


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DETERMINATION OF ANTIOXIDANT ACTIVITY OF BASIL DEFATTED SEED (Ocimum basilicum Linn.)

        Anggreini D.N. Rupidara; Kris H. Timotius; Lidya N,2017
DETERMINATION OF ANTIOXIDANT ACTIVITY OF BASIL DEFATTED SEED (Ocimum basilicum Linn.) Anggreini D.N. Rupidara1), Kris H. Timotius2), and Lidya Ninan Lestario3) Correspondent emails: adn.rupidara@gmail.com; kh_timotius@ukrida.ac.id This paper provides information on polyphenol compounds as the primary antioxidant in basil defatted seed that was collected from three locations analyzed using the Folin Ciocalteu method. Antioxidant activity is determined by using three different spectroscopic methods: Ferric thiocyanate (FTC), Diphenylpicrylhydrazyl (DPPH) and Ferric Reduced antioxidant power (FRAP) tests. The highest polyphenol content was found in Kope2 (0.65 mg), followed by Ambr1 (0.37 mg), Kupa (0.30 mg), Ambr2 (0.22 mg) and Kope1 (0.19 mg) per 100 g DW. The high antioxidant activity as shown by lowest absorbance value of the FTC row from highest to lowest insequential order i.e. Ambr2, Ambr1, Kope2, Kope1 and Kupa. DPPH mode proved that basil defatted seed has the ability to inhibit free radicals by H atom donor. The highest ability was measured at 90.88% and the lowest was 6.91%. All samples showed a positive response to reducing Ferric ions (FRAP method) with a number of capabilities 24.39% - 404.5% where 100% is equivalent to 1 mmol FeSO4. Key Words: Basil defatted seeds; Antioxidant activities; spectroscopy methods.


File Download :
2017 GCJ-GC_Antioxidant Basil Defatted seed.pdf

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